HEMOGOLOBIN ELECTROPHORESIS
1) ACCESSORIES
    - Power Supply
    - Chamber
    - Applicator Kit
    - Micro dispenser 0-10 μl
    - Micro dispenser 0-100 μl
    - Staining Set and Rack
    - Oven I . O . D .
2) REAGENTS AND CONSUMABLES
    - Acetate plates TITAN IV H
    - Super Hema buffer ( 1bag diluted in 1000 ml distilled water )
    - Ponceau S (1bag diluted in 1000 ml distilled water )
    - Clear Aid
    - Controls : - A1 A2
    - A1 SA2
    - A1FSA2
    - A1FSC
    - Hemolysate reagent
    - Report Forms
    - Wicks
    - Blotters
    - Methanol
    - Glacial acetic acid .
3) METHOD
    - Wet the plates 15mn in buffer
    - Sample preparation : 1 Part of packed red blood cells + 6 parts of hemolysate regent or 1 part
       of whole blood + 6 parts of hemolysate reagent .
    - One cathode application
    - Electrophoresis 20mn at 350 V .
4) STAINING
    - Ponceau S : 3 mn .
    - Destaining : 3 differents washes in 5 % acetic acid 3 mn each .
    - Deshydratation : 2 diffrents washes in pure methanol 2 mn each .
    - Clearing : 10 mn in the clearing solution .
    - Clearing solution ………….. 67 % pure methanol     29 % glacial acetic acid 4 % clear aid .
    - At the end of the clearing time drain the plate vertically for about 1 mn .
    - Put the plate in an oven ( acetate side up ) for 10 mn .
    - Wipe the mylar side .
5) SCANNING
    - Scan with your densitometer at 525 mn .

LIPOPROTEINS ELECTROPHORESIS
1) ACCESSORIES
    - Power Supply
    - Chamber
    - Applicator Kit
    - Micro dispenser 0-10 μl
    - Staining Set and Rack
2) REAGENTS AND CONSUMABLES
    - Acetate plates
    - HR Buffer ( 1 bag diluted in 750 ml distilled water )
    - Fat Red 7B ( 1 vial diluted in 100 ml of methanol )
    - Plastic Bags
    - Lipo Spray
    - Lipotrol ( Control )
    - Report Forms
    - Blotters
    - Glycerin
    - Methanol
    - Sodium Hydroxyde 1N .
3) METHOD
    - Wet the plates 30m in buffer
    - Sample volume : 5 µl of serum
    - 2 Superposed cathode applications
    - Electrophoresis : 20 mn at 180 V .
4) STAINING
    - Stock solution : dilute 1 vial of powder in one liter of methanol , mix for 3 to hours then filter .
    - About 5 mn before the end of the migration , mix in a dish : 30 ml of stock solution + ml of sodium
      hydroxyde 1n ; (this quantity is for one plate ) .
    - At the end of Electrophoresis time , put the plate in the staining dish 15mn (not more ) acetate side up .
    - At the end of the staining time , gently rinse the plate under tap water .
    - Then put the plate in a plastic bag with some drops of water , close it avoiding air bubbles .
5) SCANNING
    - Scan with your densitometer at 525 nm .
   
SPECIAL NOTE
    - If you want to keep the plate : put it for 10 mn in a solution composed of 80 % glacerin + 20 %
    methanol , let it drain and and dry it between two blotters .
    - Spray the cellulose acetate plate surface for 2-3 secondes and let it dry at room temperature .

LIPOPROTEINS ELECTROPHORESIS
1) ACCESSORIES
    - Power Supply
    - Chamber
    - Applicator Kit
    - Micro dispenser 0-10 μl
    - Staining Set and Rack
    - Oven I.O.D
2) REAGENTS AND CONSUMABLES
    - Acetate plates
    - HR Buffer ( 1 bag diluted in 750 ml distilled water )
    - Ponceau S (1 bag diluted in 1000 ml distilled water)
    - Clear Aid
    - Serum controls (kemtols)
    - Report Forms
    - Blotters
    - Methanol (pure)
    - Glacial acetic acid
3) METHOD
    - Wet the plates 20 mn in buffer
    - Sample volume : 3 µl of serum
    - One center application
    - Electrophoresis : 15 mn at 180 V .
4) STAINING
    - Ponceau S : 6 mn .
    - Destaining : 3 differents washes in 5 % acetic acid 3 mn each .
    - Deshydratation : 2 diffrents washes in pure methanol 2 mn each .
    - Clearing : 10 mn in the clearing solution .
    - Clearing solution ………….. 67 % pure methanol     29 % glacial acetic acid 4 % clear aid .
    - At the end of the clearing time drain the plate vertically for about 1 mn .
    - Put the plate in an oven ( acetate side up ) for 10 mn at 50c 60c
    - Wipe the mylar side .
5) SCANNING
    - Scan with your densitometer at 525 nm .